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June 1, 2024The CRISPR Journal

Establishment of a Cleavage-Based Single-Plasmid Dual-Luciferase Surrogate Reporter for the Cleavage Efficiency Evaluation of CRISPR-Cas12a Systems and Its Primary Application

Yaoqiang Shi Qi Tan Chunhui Yang Shilin Li Yujia Li Baoren He He Xie Xiaoqiong Duan Limin Chen

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Abstract

CRISPR-Cas technology is a widely utilized gene-editing tool that involves gRNA-guided sequence recognition and Cas nuclease-mediated cleavage. The design and evaluation of gRNA are essential for enhancing CRISPR/Cas editing efficiency. Various assays such as single-strand annealing,

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Cite This Study

Shi et al. (2024) studied this question.

synapsesocial.com/papers/68e672e0b6db6435875fd71c https://doi.org/10.1089/crispr.2024.0038

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