p97 inhibitor promotes apoptosis on colon cancer cells

Background: p97 is a multifunctional protein that plays an important role in DNA damage repair and processes related to programmed cell death. We used CB-5083, a specific p97 inhibitor, to evaluate the effect of inhibiting cluster proliferation and promoting apoptosis on HCT116 colon cancer cells. Materials and methods: Experimental research method used p97 inhibitor CB-5083 (Sellechem) and colon cancer cell line HCT116 (code: CCL-247, ATCC). Colony formation assay and apoptotic assay were used in this study. Data were analysed on ImageJ and GraphPad Prism 9 software. Results: The density and size of cell colonies in the 0.25 µM CB5083 treatment group were statistically significantly smaller than the other groups. The 0.25 µM treatment group had a statistically significant higher percentage of apoptotic cells compared to the 0,125µM group and the control group (p < 0.0001). The percentage of necrotic cells in the 0.125 µM and 0.25 µM treatment groups was statistically significantly higher than the control group (p < 0.0001). Conclusion: Inhibiting p97 by CB5083 suppressed cell colony formation and promoted apoptosis on HCT116 colon cancer cell line.