Meat-derived broth as a novel culture medium for metabolomic study of bacteria in meat

This is the authors' abstract. We don't add key points for this paper.

Traditional culture media often fail to mimic the natural environment of food-borne bacteria, thereby impeding accurate metabolic profiling. This study developed a novel chicken meat-derived broth (CMB) using various sterilization methods. Metabolic profiles of CMBs and bacteria were analyzed using a UHPLC-Q-Exactive Orbitrap Mass Spectrometry. Among the methods tested, the filtered and pasteurized CMB (FP-CMB) demonstrated the highest metabolomic similarity to chicken meat. FP-CMB effectively supported microbial growth without inducing changes in turbidity or contamination. The metabolites in FP-CMB played a role in altering energy synthesis and glutathione metabolism of bacteria, showcasing distinctions from those observed in commercial media. This study unveils the potential of FP-CMB as an innovative, representative, and efficient culture medium for microbiological studies, providing precise insights into bacterial metabolomics in meat products.