Inhibition Effect of Benzimidazolium Salts on Swarming Motility on Pseudomonas Aeruginosa PAO1

first_page Download PDF settings Order Article Reprints Font Type: Arial Georgia Verdana Font Size: Aa Aa Aa Line Spacing:    Column Width:    Background: Open AccessAbstract Inhibition Effect of Benzimidazolium Salts on Swarming Motility on Pseudomonas Aeruginosa PAO1 † by Müge MuşmulaMüge Muşmula SciProfiles Scilit Preprints.org Google Scholar 1,*, Ebru ÖnemEbru Önem SciProfiles Scilit Preprints.org Google Scholar 2 and Senem AkkoçSenem Akkoç SciProfiles Scilit Preprints.org Google Scholar 3,4 1 Department of Pharmacology, Faculty of Medicine, Institute of Health Sciences, Suleyman Demirel University, Isparta 32260, Türkiye 2 Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Suleyman Demirel University, Isparta 32260, Türkiye 3 Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Suleyman Demirel University, Isparta 32260, Türkiye 4 Faculty of Engineering and Natural Sciences, Bahçeşehir University, Istanbul 34000, Türkiye * Author to whom correspondence should be addressed. † Presented at the 3rd International Electronic Conference on Processes—Green and Sustainable Process Engineering and Process Systems Engineering (ECP 2024), 29–31 May 2024; Available online: https://sciforum.net/event/ECP2024. Proceedings 2024, 105(1), 5; https://doi.org/10.3390/proceedings2024105005 Published: 28 May 2024 (This article belongs to the Proceedings of The 3rd International Electronic Conference on Processes—Green and Sustainable Process Engineering and Process Systems Engineering) Download keyboard_arrow_down Download PDF Download PDF with Cover Download XML Download Epub Versions Notes Keywords: bacteria; benzimidazolium salt; P. aeruginosa; PAO1; antimicrobial activity Introduction: Bacteria can move across semisolid surfaces in colonies of flagella-driven cells. And this coordinated type of movement is called swarming motility. Swarming has been linked to increased antibiotic resistance and virulence in a variety of human infections, and it may be viewed as a beneficial adaptation to the many problems that bacteria encounter in quickly evolving settings. Pseudomonas aeruginosa is one of the species that uses swarming motility for pathogenicity. In this study, the inhibitory effect of benzimidazolium salts on swarming motility in P. aeruginosa PAO1 was investigated.Methods: P. aeruginosa PAO1 was grown overnight in Luria–Bertani medium at 37 °C. The next day, 20 mL of the molecules whose inhibitory effects were investigated were added to the swarming medium at a sub-mic concentration and poured into Petri dishes. Two µL of bacteria supernatant was inoculated in the center of the agar and incubated at 37 °C for 18–24 h. Positive control P. aeruginosa PAO1 was inoculated into the medium without adding extract. After incubation, the inhibition effect of compounds was evaluated by measuring the diameter of the swarming at the inoculation site. The study was carried out three times, and the results were compared with the control.Results: One of the virulence factors that is crucial to Pseudomonas' pathogenicity is swarming motility. Both compounds had a high rate of inhibitory effect (78%) in the swarming motility data, and all results were found to be statistically significant.Conclusions: In recent years, when antibiotic resistance has become a major problem, alternative solutions have been tried against infectious diseases. Inhibition of bacterial communication, which is important in pathogenicity, is one of the promising options. This compound may be useful in future research investigations into drug development. Author ContributionsConceptualization, S.A. and E.Ö.; methodology, M.M., E.Ö. and S.A.; software, E.Ö. and S.A.; validation, M.M., E.Ö. and S.A.; formal analysis, M.M., E.Ö. and S.A.; investigation, M.M., E.Ö. and S.A.; resources, M.M., E.Ö. and S.A.; data curation, M.M., E.Ö. and S.A.; writing—original draft preparation, M.M., E.Ö. and S.A.; writing—review and editing, M.M., E.Ö. and S.A.; visualization, E.Ö. and S.A.; supervision, E.Ö. and S.A.; project administration, E.Ö. and S.A. All authors have read and agreed to the published version of the manuscript.FundingThis research received no external funding.Institutional Review Board StatementNot applicable.Informed Consent StatementNot applicable.Data Availability StatementData will be made available by the authors if requested.Conflicts of InterestThe authors declare no conflict of interest.Disclaimer/Publisher's Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Share and Cite MDPI and ACS Style Muşmula, M.; Önem, E.; Akkoç, S. Inhibition Effect of Benzimidazolium Salts on Swarming Motility on Pseudomonas Aeruginosa PAO1. Proceedings 2024, 105, 5. https://doi.org/10.3390/proceedings2024105005 AMA Style Muşmula M, Önem E, Akkoç S. Inhibition Effect of Benzimidazolium Salts on Swarming Motility on Pseudomonas Aeruginosa PAO1. Proceedings. 2024; 105(1):5. https://doi.org/10.3390/proceedings2024105005 Chicago/Turabian Style Muşmula, Müge, Ebru Önem, and Senem Akkoç. 2024. "Inhibition Effect of Benzimidazolium Salts on Swarming Motility on Pseudomonas Aeruginosa PAO1" Proceedings 105, no. 1: 5. https://doi.org/10.3390/proceedings2024105005 Article Metrics No No Article Access Statistics Multiple requests from the same IP address are counted as one view.