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For assessment of Non-Photochemical Quenching (NPQ) relaxation in leaf discs using chlorophyll fluorescence on FluorCamFC800-331 and FluorCam 7 Software under 10 minutes of low light (50 umolm-2s-1) to activate rubisco, 15 minutes of high light (1600 umolm-2s-1) to activate NPQ without causing photodamage, and 45 minutes of NPQ relaxation under low light (50 umolm-2s-1). Fluorescence measurements using saturating pulses are taken every 2.5 minutes during high light and for the first two time points after return to low light and every 5 minutes thereafter.
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Doran et al. (2024) studied this question.
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