Multiplatform Plant Metabolomics Analysis Protocol for Arabidopsis thaliana v1

In the pursuit of comprehensive metabolomic profiling, a multiplatform analysis protocol has been developed for Arabidopsis thaliana, utilizing an array of analytical techniques to quantify a diverse set of intracellular metabolites. This protocol encompasses methods for the extraction and purification of metabolites, followed by their analysis using high-performance liquid chromatography (HPLC) for amino acids, liquid chromatography-tandem mass spectrometry (LC-MS/MS) for central carbon metabolism, and gas chromatography-tandem mass spectrometry (GC-MS/MS) for open profiling of amino acids, organic acids, sugars, and fatty acids. Each method is meticulously designed to ensure precise quantification and identification of metabolites critical for understanding the metabolic networks in Arabidopsis thaliana. This protocol not only outlines the steps for metabolite extraction and analysis but also details the preparation of reagents and solutions, instrumental settings, and the analytical conditions required for optimal detection and quantification. The integration of these platforms provides a robust framework for metabolic studies, contributing significantly to the field of plant metabolomics by enabling detailed metabolic profiling with high accuracy and reproducibility.