Study on Leukapheresis of Hyperleukocyte Acute Myeloid Leukemia

Abstract Respiratory failure, intracranial hemorrhage and infection were more common in hyperleukocyte acute myeloid leukemia patients than in non-hyperleukocyte leukemia patients. Compared with non-apheresis treatment, the white blood cells decreased significantly and the infection rate decreased after apheresis treatment. However, the treatment time of leukapheresis in patients with hyperleukocyte leukemia is very long, while the damage to cells is also large. In this study, a retrospective analysis was conducted on hyperleukocyte acute myeloid leukemia patients with hyperleukocytosis during induction. Centrifugation was performed at different rotational speeds and centrifugation times to observe whether there were changes in the number and morphology of peripheral blood cells in healthy people and patients with hyperleukocyte leukemia. The centrifugation of normal cells and hyperleukocyte was simulated in vitro, so as to explore optimal centrifugation parameters in the treatment of leukapheresis. The cells obtained by optimal centrifugation parameters were cryopreserved and animal models were established. Through the research, it is found that when the rotational speed is increased below 6000rpm, the damage to normal blood cells and blood cells in patients with hyperleukocyte leukemia is small. When the rotational speed is greater than 6000rpm, the platelets will be damaged. The cells obtained under optimal centrifugation parameters can be successfully cryopreserved and modeled in leukemia animals.