Enterovirga aerilata sp. nov. and Knoellia koreensis sp. nov., isolated from an automobile air conditioning system

Two strictly aerobic and rod-shaped bacteria, labelled as DB1703 T and DB2414S T , were obtained from an automobile air conditioning system. Strain DB1703 T was Gram-stain-negative, while strain DB2414S T was Gram-stain-positive. Both strains were catalase-positive and oxidase-negative. Strains DB1703 T and DB2414S T were able to grow at 18–42 °C. Strain DB1703 T grew within a NaCl range of 0–3 % and a pH range of 6.0–8.0; while strain DB2414S T grew at 0–1 % and pH 6.5–8.5. The phylogenetic and 16S rRNA gene sequence analysis indicated that strains DB1703 T and DB2414S T belonged to the genera Enterovirga and Knoellia , respectively. Strain DB1703 T showed the closest phylogenetic similarity to Enterovirga rhinocerotis YIM 100770 T (94.8 %), whereas strain DB2414S T was most closely related to Knoellia remsis ATCC BAA-1496 T (97.7 %). The genome sizes of strains DB1703 T and DB2414S T were 4 652 148 and 4 282 418 bp, respectively, with DNA G+C contents of 68.8 and 70.5 mol%, respectively. Chemotaxonomic data showed Q-10 as the sole ubiquinone in DB1703 T and ML-8 (H4) in DB2414S T . The predominant cellular fatty acid in DB1703 T was summed feature 8 (C 18 : 1 ω 7 c and/or C 18 : 1 ω 6 c ), whereas iso-C 16 : 0 , C 17 : 1 ω 8 c , and iso-C 15 : 0 were dominant in DB2414S T . Overall, the polyphasic taxonomic comparisons showed that strains DB1703 T and DB2414S T were distinct from their closest taxa and represent novel species within the genera Enterovirga and Knoellia , respectively. Accordingly, we propose the names Enterovirga aerilata sp. nov., with the type strain DB1703 T (=KCTC 72724 T =NBRC 114759 T ), and Knoellia koreensis sp. nov., with the type strain DB2414S T (=KCTC 49355 T =NBRC 114620 T ).