Establishment and Maintenance of Organotypic Cerebellar Slice Cultures (OCerSC) from Aged Mice v1

This protocol outlines a method for culturing organotypic slices from aged mouse cerebellar tissue, maintaining the cerebellum’s complex three-dimensional structure and cellular composition ex vivo. This technique simulates various physiological and pathophysiological conditions to study neurodevelopmental and neurodegenerative disorders and synaptic plasticity mechanisms. Expected outcomes include detailed neuronal morphology and functional connectivity analyses at the cellular and circuit levels using advanced imaging, electrophysiological techniques, and biochemical analyses such as protein expression, gene expression, metabolic activity, oxidative stress markers, neurotransmitter levels, calcium imaging, and enzyme activity assays.