Studying the Relationship Between Seminal Plasma Enzymes and Chemical Elements Concentration With the Physical Semen Properties of Local Awassi Rams

Semen samples were collected by using 10 adult Awassi Rams biweekly via electric ejaculator in one ejaculate/ram. Semen properties, enzymes concentration (AST, ALT, ALP, LDH, ACP), elements concentration (Na, K, Ca, Zn, Cl, P, Mg and Fe) in semen, were determined. The results showed that AST enzyme is significantly correlated (r = -0.671),(r = -0.500),(r = -0.587),(r = 0.587) with pH, individual motility, live and dead sperms. ALT enzyme was significantly correlated (r = -0.578),(r = -0.560), (r = 0.560) with pH, live and dead sperms respectively. A negative correlation was found between ALP and semen volume, pH, mass and individual motility, live sperm and sperm membrane integrity, while there was a positive correlation with the sperms concentration and dead and abnormal sperms. LDH enzyme showed a positive correlation with semen volume, mass and individual motility, live and abnormal sperms. A negative correlation with pH, sperms concentration, dead sperm and sperm membrane integrity. ACP enzyme had a positive correlation with volume, mass and individual motility, sperms concentration, dead sperms and sperm membrane integrity. Na and Cl were significantly correlated with semen volume, and Cl with mass motility. Zn, Ca, Mg and Fe were positively and significantly correlated with sperms concentration. A positive significant correlation was found between Ca and individual motility, live and sperms concentration, while this correlation was significantly negative with dead and abnormal sperms. Therefore, the enzymes and minerals status of the seminal fluids are considered an essential index for evaluating the quality of Awassi rams semen.