FIGURE 2 from Urolithin-A Promotes CD8⁺ T Cell–mediated Cancer Immunosurveillance via FOXO1 Activation

UroA promotes naïve T-cell expansion in vivo and memory formation in vitro.A, Schematic representation of in vivo feeding experiment. B, Frequency of naïve CD8⁺ T cells (CD62L⁺CD44⁻) isolated from the spleen of mice fed with control or UroA-enriched diet. C, Expression level of CD62 L in CD8⁺ T cells from B, values are normalized to the control. D, Frequency of central memory (CD62L⁺CD44⁺) in CD8⁺ T cells treated for 4 days with 5 µmol/L UroA in IL15/7 condition in vitro. E, Expression level of central memory marker CD62 L in C. F, Analysis of tumor growth rate of B16-Ova tumor-bearing mice adoptively transferred with no cells (saline = 13 mice), control OT-1 CD8⁺ T cells (control = 10 mice) and UroA-treated OT-1 CD8⁺ T cells (UroA = 13 mice). G, Quantification of expression level of TIM-3 and PD-1 in TILs from F measured (control = 7, UroA = 6). H, Frequency of terminally exhausted T cells (TCF1⁻PD1⁺TIM3⁺) from G. Data are mean ± SEM. Each dot represents a biological replicate. In B and C, control n = 10 mice, UroA = 12 mice. In D and E, sample size n = 5. Data were analyzed by two-sided Student t test (*, P < 0.05; **, P < 0.01). Representative results of two independent experiments or two pooled experiments.