This procedure provides instructions for how to perform qualitative PCR (qPCR) for the detection of the IS481 insertion sequence of Bordetella pertussis and the pIS1001 insertion sequence of the Bordetella parapertussis from appropriate direct specimens. The IS481 insertion has been found to be present in some strains of B. bronchiseptica and most strains of B. holmesii. Some strains of B. bronchseptica also carry the pIS1001insertion sequence. The B. holmesii and B. bronchiseptica multiplex qPCR should be performed when indicated.
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Cheung et al. (2024) studied this question.
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