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This is an RNA extraction method that isolates HPAI RNA segments from commercially-available milk samples. The protocol involves processing with a MagMax RNA extraction kit on a Kingfisher instrument. Then, the samples are concentrated using a Zymo Clean and Concentrate kit. The total RNA yields should be suitable for performing qPCR. After trying other methods involving centrifugation, filtration, and trizol, we determined this method to be the easiest and most effective.
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Lail et al. (2024) studied this question.
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