Isolation of Carbohydrate Hydrolyzing Enzymes from Red Kidney Beans

Humans and other monogastric animals lack certain glycosidase enzymes that are widely present in plant seeds and animal tissues. This study aimed to extract glycosidase enzymes from red kidney bean seeds. The optimal germination period was found to be 6 days, resulting in the extraction of approximately 230.0 g of cotyledons. Analysis revealed the presence of six forms of glycosidase enzymes: α- and β-glucosidase, α- and β-galactosidase, and α- and β-mannosidase. The protein concentration in the extract was determined to be 0.271 mg protein ml-1. Enzyme activities were measured for each form: α-glucosidase (1.90 mM min-1 ml-1), β-glucosidase (4.10 mM min-1 ml-1), α-galactosidase (0.76 mM min-1 ml-1), β-galactosidase (1.70 mM min-1 ml-1), α-mannosidase (2.07 mM min-1 ml-1), and β-mannosidase (0.90 mM min-1 ml-1). Specific activities were also calculated: α-glucosidase (3.76 mM min-1 mg protein-1), β-glucosidase (8.10 mM min-1 mg protein-1), α-galactosidase (1.55 mM min-1 mg protein-1), β-galactosidase (3.31 mM min-1 mg protein-1), α-mannosidase (4.25 mM min-1 mg protein-1), and β-mannosidase (1.90 mM min-1 mg protein-1). Study highlights the high activity levels of carbohydrate hydrolysis enzymes (glycosidases). In red kidney bean plants. The findings emphasize the importance of red kidney bean seeds as rich source of these biocatalysts with diverse applications.