First Report in Korea of Vibrio spp. Observed during the Artificial Seeding Production Process of the Giant Pacific Octopus, Enteroctopus Dofleini

We are conducting research on the artificial seeding production of the giant Pacific octopus (Enteroctopus dofleini) for the first time in Korea, and studies have been conducted on the Vibrio spp. that occur during this process. In the artificial seeding production process of the giant Pacific octopus, Vibrio splendidus, V. chagasii, V. kanaloae, and V. pelagius were detected in the mortalities of giant Pacific octopus paralarvae and Artemia. It is widely known that V. splendidus is mostly isolated from Pacific oysters (Crassostrea gigas), and there have been no reports of its discovery in giant Pacific octopus paralarvae. V. chagasii, V. kanaloae, and V. pelagius have been reported to belong to the clade of V. splendidus. After the challenge experiment the survival rate of giant Pacific octopus paralarvae in the group injected with V. splendidus without antibiotic treatment sharply decreased to the 50% range on days 2. In contrast, the group treated with antibiotics and injected with V. splendidus showed a survival rate of over 80% on days 2. Vibrio spp. were observed in both Artemia and giant Pacific octopus paralarvae, however, research is deemed necessary to investigate the impact of these Vibrio spp. on giant Pacific octopus paralarvae in the future.