Genetic Improvement of Some Trichoderma Isolates for Second-Generation Biofuel Production from Agricultural Wastes.

Twenty-six isolates of Trichoderma spp. collected from various Egyptian locations were screened for their Cellulase activities via qualitative and quantitative tests. The main objective was to choose the best isolates that can degrade lignocellulosic biomass. First, we carried out qualitative screening of Cellulases in Congo red dye medium. Six isolates showed clear zones larger than other isolates namely, MNF-MAS-Tricho1, 6, 23, and ASI-MAS-Tricho26. In addition, another qualitative test was done using 1% PASC media. Full plate radial growth was shown in isolates MNF-MAS-Tricho 2 ,3 ,5 ,6 ,12 ,15 ,18 ,21 , and 23 after 48 h incubation and the sporulation type was detected