Comparison of the ForenSeq Signature Prep and ForenSeq MainstAY kits to traditional capillary electrophoresis kits for STR profile analysis from various DNA sources

Comparisons of DNA profiles recovered from the crime-scene and a person through short tandem repeat (STR) analysis using capillary electrophoresis (CE) is the gold standard method of determining a match. A comparison of STR profiles generated using CE to massively parallel sequencing (MPS) methods was undertaken. The results showed CE methods recovered high percentages of autosomal or Y-STR markers individually, however, MPS produced more information at low inputs and a higher number of STR markers overall, showing the potential of MPS to provide additional data to assist in solving major crimes.