Label-free sample analysis using digital holographic microscopy

Digital holographic microscopy (DHM) is a label- free quantitative imaging technique to image and characterize translucent to transparent samples. It uses coherent principles of the light source for interferometric detection of transparent and semi- transparent samples. The coherence property of the laser source leads to speckles which often act as noise. Laser speckle contrast imaging is a non-destructive tool for full field mapping of flow in the field of view. It can be integrated with off-axis DHM for applications in optofluidics. This enables the mapping of flow within a microfluidic channel and quantifying the channel dimensions as well as the particles flowing through the channel.