Mp59-19 Mitochondrial Morphology in the External Urethral Sphincter: Implications for Muscle Dysfunction and Urinary Incontinence

You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Basic Research & Pathophysiology (MP59)1 May 2024MP59-19 MITOCHONDRIAL MORPHOLOGY IN THE EXTERNAL URETHRAL SPHINCTER: IMPLICATIONS FOR MUSCLE DYSFUNCTION AND URINARY INCONTINENCE Shinro Hata, Mayuka Shinohara, Hiromitsu Mimata, and Toshitaka Shin Shinro HataShinro Hata , Mayuka ShinoharaMayuka Shinohara , Hiromitsu MimataHiromitsu Mimata , and Toshitaka ShinToshitaka Shin View All Author Informationhttps://doi.org/10.1097/01.JU.0001009476.18935.3b.19AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: In healthy striated muscle, mitophagy, a form of selective autophagy, plays a crucial role in degrading and removing damaged mitochondria via lysosomes, thereby preserving muscle tissue homeostasis. It is widely accepted that a decline in autophagy activity with aging contributes to a decrease in muscle mass. However, there has been a lack of comprehensive investigations into the detailed morphology of mitochondria within the external urethral sphincter muscle in elderly human subjects. This study aimed to identify abnormalities in mitochondrial morphology within the external urethral sphincter by transmission electron microscopy (TEM). METHODS: To elucidate the nature of mitochondrial abnormalities in the external urethral sphincter, we employed TEM to observe mitochondrial morphology. Tissue samples from the external urethral sphincter of eight patients were obtained during radical cystectomy procedures at our institution. For comparison, rectus abdominis muscle tissue was collected. Both sets of samples were examined using hematoxylin and eosin (HE) staining and TEM. TEM images were captured and the area, perimeter, and luminance of mitochondria were measured using the ROI manager in ImageJ software (NIH). Statistical examination was conducted using the Student's t-test, with p<0.05 being considered statistically significant. RESULTS: In HE-stained images, the external urethral sphincter tissue exhibited a lower proportion of muscle fibers and a higher amount of interstitial tissue in comparison to rectus abdominis muscle tissue. TEM images of the external urethral sphincter revealed more gaps in the muscle bundles, fewer mitochondria, and structural anomalies compared to rectus abdominis muscle tissue. Furthermore, a quantitative analysis of mitochondrial morphology demonstrated that the area, perimeter, and luminance of mitochondria in the external urethral sphincter tissue were greater in size, longer in perimeter, and higher in luminance compared to the rectus abdominis muscle tissue (p<0.01) (Table 1). CONCLUSIONS: Our findings suggest that mitochondrial abnormalities in the external urethral sphincter of elderly individuals may contribute to muscle dysfunction and urinary incontinence. Source of Funding: None © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e965 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Shinro Hata More articles by this author Mayuka Shinohara More articles by this author Hiromitsu Mimata More articles by this author Toshitaka Shin More articles by this author Expand All Advertisement PDF downloadLoading ...