Mp05-06 Therapeutic Effects of Hyperthermia Using a Novel Iron Oxide Nanoparticle With Carbon Nanohorn in Castration-Resistant Prostate Cancer in a Xenograft Mouse Model

You have accessJournal of UrologyProstate Cancer: Basic Research & Pathophysiology I (MP05)1 May 2024MP05-06 THERAPEUTIC EFFECTS OF HYPERTHERMIA USING A NOVEL IRON OXIDE NANOPARTICLE WITH CARBON NANOHORN IN CASTRATION-RESISTANT PROSTATE CANCER IN A XENOGRAFT MOUSE MODEL Takashi Nagai, Noriyasu Kawai, Nobuhiko Shimizu, Toshiki Etani, Taku Naiki, Shuzo Hamamoto, Atsushi Okada, Kaname Tsutsumiuchi, Toyoko Imae, and Takahiro Yasui Takashi NagaiTakashi Nagai , Noriyasu KawaiNoriyasu Kawai , Nobuhiko ShimizuNobuhiko Shimizu , Toshiki EtaniToshiki Etani , Taku NaikiTaku Naiki , Shuzo HamamotoShuzo Hamamoto , Atsushi OkadaAtsushi Okada , Kaname TsutsumiuchiKaname Tsutsumiuchi , Toyoko ImaeToyoko Imae , and Takahiro YasuiTakahiro Yasui View All Author Informationhttps://doi.org/10.1097/01.JU.0001008740.27639.cc.06AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Castration-resistant prostate cancer typically exhibits a poor prognosis and limited treatment options, highlighting the urgent need for new therapies. To address this issue, we have adopted magnetic hyperthermia for the treatment of prostate cancer by developing a novel magnetic nanoparticle. METHODS: We synthesized a novel nanoparticle by combining carbon nanohorn (CNH) and iron oxide nanoparticle (IONP), referred to as CNH-IONP (Figure 1). Subcutaneous implantation of a human castration-resistant prostate cancer cell line, 22Rv1, was performed in the backs of six-week-old male nude mice. We organized the mice into three groups: the control group (n=6), the injection group (administered CNH-IONP via injection), and the treatment group (administered CNH-IONP + AMF irradiation). At three weeks (day 21) post-implantation, 300 μL of CNH-IONPs (Fe concentration: 36.3 mg/mL) was locally injected into the tumors of mice in the injection and treatment groups. Tumor sizes were monitored over time to assess the impact of hyperthermia. RESULTS: Mice in the treatment group underwent AMF irradiation from a coiled AMF generator for 30 minutes every two days (Figure 2). The temperature within tumors of the treatment group was maintained between 42°C and 46°C, defining a state of hyperthermia. The growth ratio of tumors in the treatment group exhibited significant suppression compared to the other groups (p<0.01, Kruskal–Wallis test). CONCLUSIONS: Through the development of the novel nanoparticle, CNH-IONP, we were able to effectively suppress tumor progression in a mouse model of metastatic prostate cancer, demonstrating the potential of this approach in hyperthermia therapy. Download PPTDownload PPT Source of Funding: None © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e44 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Takashi Nagai More articles by this author Noriyasu Kawai More articles by this author Nobuhiko Shimizu More articles by this author Toshiki Etani More articles by this author Taku Naiki More articles by this author Shuzo Hamamoto More articles by this author Atsushi Okada More articles by this author Kaname Tsutsumiuchi More articles by this author Toyoko Imae More articles by this author Takahiro Yasui More articles by this author Expand All Advertisement PDF downloadLoading ...