MP44-06 RENOPROTECTIVE EFFECT OF PDE5i IN A PARTIALLY NEPHRECTOMIZED RAT MODEL

You have accessJournal of UrologyKidney Cancer: Localized: Surgical Therapy III (MP44)1 May 2024MP44-06 RENOPROTECTIVE EFFECT OF PDE5i IN A PARTIALLY NEPHRECTOMIZED RAT MODEL Jae Sung Lim, Oh Jun Yun, Jae Hoon Kang, Sang Hee Lee, Minji Jeon, Eun Hye Lee, Jeong Seok Oh, Jun-Koo Kang, Yun-Sok Ha, Seock Hwan Choi, So Young Chun, Jun Nyung Lee, Bum Soo Kim, Hyun Tae Kim, Tae-Hwan Kim, Eun Sang Yoo, Tae Gyun Kwon, and Jae-Wook Chung Jae Sung LimJae Sung Lim , Oh Jun YunOh Jun Yun , Jae Hoon KangJae Hoon Kang , Sang Hee LeeSang Hee Lee , Minji JeonMinji Jeon , Eun Hye LeeEun Hye Lee , Jeong Seok OhJeong Seok Oh , Jun-Koo KangJun-Koo Kang , Yun-Sok HaYun-Sok Ha , Seock Hwan ChoiSeock Hwan Choi , So Young ChunSo Young Chun , Jun Nyung LeeJun Nyung Lee , Bum Soo KimBum Soo Kim , Hyun Tae KimHyun Tae Kim , Tae-Hwan KimTae-Hwan Kim , Eun Sang YooEun Sang Yoo , Tae Gyun KwonTae Gyun Kwon , and Jae-Wook ChungJae-Wook Chung View All Author Informationhttps://doi.org/10.1097/01.JU.0001009508.69111.d0.06AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: To date, there is no definitive treatment for preventing renal ischemia-reperfusion (IR) injury during partial nephrectomy (PN). Futhermore, there are no animal models on renal IR injury during PN. This study aims to develop an optimal animal model for renal IR injury that occurs during PN and analyze the efficacy and safety of phosphodiesterase type 5 inhibitor (PDE5i)'s renal protective function. METHODS: Animal model of renal IR injury was established via PN and oral administration of daily tadalafil (5 mg/kg) to 6-week-old male mice for 2 weeks before PN. The degree of functional, histological, and molecular injury was analyzed through blood and kidney tissue. RESULTS: There was no significant difference in serum creatinine and the glomerular filtration rate between tadalafil group and control group. However, histologically, the number of glomerulus increased significantly in tadalafil group. Massons' Trichrome staining confirmed that fibrosis, which increased in the renal IR group, was partially suppressed in the tadalafil group. Immunologic analysis showed that expression of TNF-α, IL-1β, IL-6, iNOS, and PDE5 reduced significantly in tadalafil group. Molecular biological analysis showed that expression of kidney MPO reduced significantly in tadalafil group. Immunohistochemistry (IHC) confirmed that T cells, which were increased in the renal IR injury group, were partially reduced in the tadalafil group through CD4 (T helper cell) and CD8 (cytotoxic T cell) makers. And also IHC showed that apoptosis and inflammatory responses through p53 and eNOS were partially reduced in the tadalafil group. CONCLUSIONS: We developed optimal animal model of renal IR injury during PN and verified renal protective effect of PDE5i. Source of Funding: Nothing to disclose © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e732 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Jae Sung Lim More articles by this author Oh Jun Yun More articles by this author Jae Hoon Kang More articles by this author Sang Hee Lee More articles by this author Minji Jeon More articles by this author Eun Hye Lee More articles by this author Jeong Seok Oh More articles by this author Jun-Koo Kang More articles by this author Yun-Sok Ha More articles by this author Seock Hwan Choi More articles by this author So Young Chun More articles by this author Jun Nyung Lee More articles by this author Bum Soo Kim More articles by this author Hyun Tae Kim More articles by this author Tae-Hwan Kim More articles by this author Eun Sang Yoo More articles by this author Tae Gyun Kwon More articles by this author Jae-Wook Chung More articles by this author Expand All Advertisement PDF downloadLoading ...