Mp63-09 Oxalate Metabolism Is Highly Redundant in the Human Gut Microbiota

You have accessJournal of UrologyStone Disease: Basic Research & Pathophysiology (MP63)1 May 2024MP63-09 OXALATE METABOLISM IS HIGHLY REDUNDANT IN THE HUMAN GUT MICROBIOTA Sromona D. Mukherjee, Mangesh Suryavanshi, and Aaron W. Miller Sromona D. MukherjeeSromona D. Mukherjee , Mangesh SuryavanshiMangesh Suryavanshi , and Aaron W. MillerAaron W. Miller View All Author Informationhttps://doi.org/10.1097/01.JU.0001009436.52988.91.09AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: The human gut microbiota, comprising a complex community of microorganisms, plays a pivotal role in human health, including nutrient metabolism, immune system modulation, and protection against pathogenic invaders. Research shows interactions between the gut microbiota and dietary components, such as oxalate, a dietary toxin. Oxalate, found in a variety of foods when accumulated is detrimental, leading to kidney stones–affecting millions worldwide. This study assessed the prevalence and abundance of genes associated with oxalate metabolism, specifically related to oxalate degradation or associated with acetogenic, methanogenic or sulfate reducing (AMS) genes, within the gut microbiota along with phylogenetic analysis of the frc gene. METHODS: Full-length genomes were obtained from a cohort of 35 healthy individuals and 35 patients with Urinary stone Disease (USD). Genomes underwent gene extraction and annotation using PROKKA. The selection of genes was based on KEGG metabolic pathways. The phylogenetic tree was generated using the Reltime algorithm. RESULTS: 32.5% of genomes were found to contain at least one oxalate-degrading gene. In contrast the number of genomes harboring at least one gene in the AMS pathways exceeded 95%. Importantly, individuals with USD harbored lower abundances of bacterial genomes associated with oxalate and AMS pathways particularly bacteria from the Faecalibacterium genus. The phylogenetic tree indicates that the frc gene has its origins in the genus Bradyrhizobium, whereas Oxalobacter formigenes exhibits a recent, but rapid emergence as an oxalate-degrader. CONCLUSIONS: The study's findings (Fig 1), shed light on the metabolic pathways involving oxalate, acetogenic, methanogenic, and sulfate-reducing processes in humans. The high degree of metabolic redundancy in the oxalate-degrading pathway is in contrast to the hypothesis that Oxalobacter formigenes is primarily responsible for oxalate homeostasis. The universal prevalence of AMS pathways indicates that formate metabolism, a by-product of oxalate degradation and other metabolic pathways, is critical for the survival of bacteria in the gut environment. Phylogenetic analysis data aligns with the hypothesis that Oxalobacter formigenes harbors a recent, derivative form of the oxalate-degrading gene. Download PPT Source of Funding: None © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e1034 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Sromona D. Mukherjee More articles by this author Mangesh Suryavanshi More articles by this author Aaron W. Miller More articles by this author Expand All Advertisement PDF downloadLoading ...