Pd09-01 Regulatory Effects of Mir-21 on the Prostate With or Without Tumor-Bearing Conditions: A Novel Perspective Based on Extracellular Vesicles

You have accessJournal of UrologyProstate Cancer: Basic Research & Pathophysiology II (PD09)1 May 2024PD09-01 REGULATORY EFFECTS OF MIR-21 ON THE PROSTATE WITH OR WITHOUT TUMOR-BEARING CONDITIONS: A NOVEL PERSPECTIVE BASED ON EXTRACELLULAR VESICLES Cong Hu, Polina Sysa-Shah, Xinrui Wu, Sarah R. Amend, Wei Xue, Shawn E. Lupold, Kenneth J. Pienta, and Liang Dong Cong HuCong Hu , Polina Sysa-ShahPolina Sysa-Shah , Xinrui WuXinrui Wu , Sarah R. AmendSarah R. Amend , Wei XueWei Xue , Shawn E. LupoldShawn E. Lupold , Kenneth J. PientaKenneth J. Pienta , and Liang DongLiang Dong View All Author Informationhttps://doi.org/10.1097/01.JU.0001008572.33286.63.01AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Most studies have shown a negative correlation between miR-21 and the prognosis of prostate cancer (PCa) patients. Extracellular vesicles (EVs) play a pivotal role as a means of cell-to-cell communication at all stages of disease. Whether miR-21 can regulate extracellular vesicular contents to influence disease turnover is not elucidated. METHODS: In this study we separated EVs from ex vivo prostate tissue and urine of four mice models and performed mRNA profiling using the NanoString nCounter. Wild-type (WT) WT with miR-21 knockout (WT-miR21 KO), Hi-Myc transgenic (TG) model of PCa (Hi-Myc TG) and Hi-Myc TG with miR-21 knockout (Hi-Myc TG-miR21 KO) were used to investigate the regulation of EVs-containing mRNAs by miR-21 in physiological and pathological conditions of the prostate. RESULTS: Overall, tissue-derived EVs are more responsive to alterations mediated by miR-21 than urine EVs. More proliferation-associated mRNAs were enriched in EVs from Hi-Myc TG than WT. We found that miR-21 is capable of exerting indispensable effects on gland development and morphological maintenance of prostate with or without tumor-bearing conditions. More differentially carried transcripts in EVs were found when comparing Hi-Myc TG with Hi-Myc TG-miR21 KO, versus the comparison between WT and WT-miR21 KO, demonstrating miR-21 may play a more important role in PCa than in normal prostate, including but not limited to the modulation of drug resistance related to platinum and EGFR tyrosine kinase inhibitor. EV-derived Fen1 was identified to be a potential target to block the miR21-mediated cancer proliferation. CONCLUSIONS: Our results elucidate the effects exerted by miR-21 in prostate with or without tumor-bearing conditions based on EV mRNA profiling for the first time. Download PPT Source of Funding: NCI grants U54CA143803, CA163124, CA093900, and CA143055, and the Prostate Cancer Foundation © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e179 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Cong Hu More articles by this author Polina Sysa-Shah More articles by this author Xinrui Wu More articles by this author Sarah R. Amend More articles by this author Wei Xue More articles by this author Shawn E. Lupold More articles by this author Kenneth J. Pienta More articles by this author Liang Dong More articles by this author Expand All Advertisement PDF downloadLoading ...