Pd03-08 the Activated Complement Pathway in the Fibrous Process of Benign Prostatic Hyperplasia

You have accessJournal of UrologyBenign Prostatic Hyperplasia: Basic Research & Pathophysiology (PD03)1 May 2024PD03-08 THE ACTIVATED COMPLEMENT PATHWAY IN THE FIBROUS PROCESS OF BENIGN PROSTATIC HYPERPLASIA Junya Hata, Yuki Harigane, Kei Yaginuma, Syunta Makabe, Hitomi Imai, Seiji Hoshi, Yuichi Sato, Hidenori Akaihata, Soichiro Ogawa, and Yoshiyuki Kojima Junya HataJunya Hata , Yuki HariganeYuki Harigane , Kei YaginumaKei Yaginuma , Syunta MakabeSyunta Makabe , Hitomi ImaiHitomi Imai , Seiji HoshiSeiji Hoshi , Yuichi SatoYuichi Sato , Hidenori AkaihataHidenori Akaihata , Soichiro OgawaSoichiro Ogawa , and Yoshiyuki KojimaYoshiyuki Kojima View All Author Informationhttps://doi.org/10.1097/01.JU.0001009388.01015.e5.08AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: In previous study, we have reported that complement activation by autoimmune reaction was associated with the growth process of benign prostatic hyperplasia (BPH). On the other hand, BPH was reported to show a histological change to fibrous-dominant findings as it becomes more severe. Therefore, we hypothesized that activation of complement pathways might be associated with the fibrous process of BPH with this aggravation. In this study, to elucidate the changes in activated complement pathway in the fibrous process of BPH, we analyzed the correlation between complement component expression and histological types of fibrosis using human BPH tissue. METHODS: Fifty-six histological BPH patients who underwent prostate needle biopsy at our institution (mean age 68.6±6.5 years), divided into two histological groups, fibromuscular and fibrous, were compared. Inflammatory cell infiltration in BPH tissue were evaluated by immunohistochemical staining using CD45, with complement expression analysis performed using C3, factor B, and C5b-9 antibody, and the occupancy ratio of the stained region was calculated. Further, correlation between the histological types of fibrous components in BPH tissue and lower urinary tract symptoms questionnaires was analyzed. RESULTS: Twenty-seven (48.2%) and 29 (51.8%) cases were classified in the fibromuscular and fibrous groups, respectively. The proportion of CD45-positive cells in BPH tissue was significantly higher in the fibromuscular group. In complement component analysis, factor B did not significantly differ between groups, while C3 (fibromuscular group; 10.7±8.2%, fibrous group; 16.4±12.7%) and C5b-9 (fibromuscular group; 15.9±6.2%, fibrous group; 17.6±9.2%) were significantly higher in the fibrous group (p=0.04, p=0.04, respectively). International Prostate Symptom Score Q5 subscore, indicating slow stream, was significantly higher in the fibrous group (p=0.04). CONCLUSIONS: In fibrous BPH with abundant fibrosis, the late complement pathway in addition to alternative pathway was activated compared to fibromuscular BPH. These results suggested that the alternative and late complement pathways was involved in the histological fibrous process of BPH. Source of Funding: None © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e79 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Junya Hata More articles by this author Yuki Harigane More articles by this author Kei Yaginuma More articles by this author Syunta Makabe More articles by this author Hitomi Imai More articles by this author Seiji Hoshi More articles by this author Yuichi Sato More articles by this author Hidenori Akaihata More articles by this author Soichiro Ogawa More articles by this author Yoshiyuki Kojima More articles by this author Expand All Advertisement PDF downloadLoading ...