V07-03 Fine Needle Aspiration Mapping of the Testis for Nonobstructive Azoospermia: A Step-by-Step Clinic-Based Procedure

You have accessJournal of UrologySexual Dysfunction/Infertility/Andrology (V07)1 May 2024V07-03 FINE NEEDLE ASPIRATION MAPPING OF THE TESTIS FOR NONOBSTRUCTIVE AZOOSPERMIA: A STEP-BY-STEP CLINIC-BASED PROCEDURE Alvaro Santamaria, Tristan Nicholson, Arash Amighi, Theodore Crisostomo-Wynne, Charles Muller, Sarah Holt, Thomas Walsh, and Marah Hehemann Alvaro SantamariaAlvaro Santamaria , Tristan NicholsonTristan Nicholson , Arash AmighiArash Amighi , Theodore Crisostomo-WynneTheodore Crisostomo-Wynne , Charles MullerCharles Muller , Sarah HoltSarah Holt , Thomas WalshThomas Walsh , and Marah HehemannMarah Hehemann View All Author Informationhttps://doi.org/10.1097/01.JU.0001008932.49144.fd.03AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: The identification and usage of testicular sperm in men with azoospermia is a resource intensive process. Fine needle aspiration (FNA) mapping of the testis is a diagnostic procedure that detects the presence and relative quantity of sperm with spatial precision. Furthermore, upon identifying sperm via mapping, that information can then be used to help guide the type of surgical sperm retrieval that follows. In this video we present a patient who underwent FNA mapping, show a step by step on how to obtain specimens and process them to then be examined under the microscope. We also provide FNA diagnosis results from a retrospective review using this process. METHODS: With patient consent, we recorded an FNA mapping procedure that was performed under local anesthesia in a procedure room. We then stained the specimens under a fume hood and later examined them under a microscope. We also completed a retrospective review of 220 men with azoospermia who underwent FNA mapping between 2009 and May 2023. Their clinical data including subsequent sperm extraction technique and success were reviewed. RESULTS: In this video we successfully show how to safely perform FNA mapping under local anesthesia. We then showed how to process FNA slides and stain them for microscopic examination. Results from our retrospective review show that out of 220 men who underwent an FNA mapping, 84 (38%) were found to have sperm. Of those who then decided to undergo surgical sperm extraction, all had successful sperm retrievals. CONCLUSIONS: In conclusion, FNA mapping is an in-clinic procedure done under local anesthesia with a low complication rate. FNA mapping helps avoid high-cost procedures and helps guide what type of surgical sperm extraction technique is suitable based on FNA diagnosis. Download PPT Source of Funding: None © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e467 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Alvaro Santamaria More articles by this author Tristan Nicholson More articles by this author Arash Amighi More articles by this author Theodore Crisostomo-Wynne More articles by this author Charles Muller More articles by this author Sarah Holt More articles by this author Thomas Walsh More articles by this author Marah Hehemann More articles by this author Expand All Advertisement PDF downloadLoading ...