Mp69-12 Initial Antimicrobial Testing of a Novel Reusable Intermittent Urinary Catheter System and Catheter Reprocessing Device

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Kidney & Bladder I (MP69)1 May 2024MP69-12 INITIAL ANTIMICROBIAL TESTING OF A NOVEL REUSABLE INTERMITTENT URINARY CATHETER SYSTEM AND CATHETER REPROCESSING DEVICE Alyssa La Bella, Alex Molesan, Daniel A. Wollin, Souvik Paul, and Ana L. Flores-Mireles Alyssa La BellaAlyssa La Bella , Alex MolesanAlex Molesan , Daniel A. WollinDaniel A. Wollin , Souvik PaulSouvik Paul , and Ana L. Flores-MirelesAna L. Flores-Mireles View All Author Informationhttps://doi.org/10.1097/01.JU.0001008892.86171.de.12AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Individuals with neurogenic bladder often require single-use intermittent catheters (IC) to empty their bladders. Urinary tract infection (UTI) is a common cause of morbidity in this population, yet IC users frequently reuse single-use ICs due to financial reasons, increasing UTI risk. The Aurie No-Touch Reusable Catheter System is a preclinical prototype that allows for standardized IC reuse utilizing a proprietary device and process to remove bacteria and soiling from Aurie Catheters (ACs). This study attempted to quantify cleaning and disinfection capability of the prototype system. METHODS: Standard ICs were inoculated for 5 minutes with 106 colony forming units of uropathogenic E. coli, washed with water, and incubated at 3, 6, 9, 24, 48 hours to assess attachment (with sonication in PBS and plating) and biofilm formation (utilizing bacterial-targeted rabbit antibodies and donkey anti-rabbit IRDye 800CW secondary antibodies, allowing for infrared biofilm imaging). This procedure was repeated with ACs, both with catheters reprocessed after inoculation/incubation and catheters that were not reprocessed. This procedure was also repeated with ACs with 20 inoculation/incubation/reprocessing cycles compared to ACs with the last reprocessing step omitted to assess for change with repetitive reprocessing. This was also completed up to 101 cycles. RESULTS: The standard catheters showed bacterial attachment and biofilm development peaking at 24 hours of incubation after a 5-minute inoculation. ACs produced a similar outcome but, after reprocessing them, all pathogen loads were reduced below the level of detection (Figure 1). Repeat colonization and 20 reprocessing cycles showed notable pathogen clearance to below levels of detection. While only a single catheter reached 101 cycles, there was no viable pathogen load after reprocessing. CONCLUSIONS: Intermittent urinary catheters, when cleaned inappropriately, can harbor bioburden as viable bacteria and biofilm. The Aurie No-Touch Reusable Catheter, when cleaned and disinfected within a prototype reprocessing device, has the capacity to reduce bioburden below the level of detection, despite 101 reprocessing cycles. This suggests the Aurie System may be a feasible technology for safe catheter reuse. Download PPT Source of Funding: This project was supported by the NSF 21-565 SBIR Phase II grant # 2147852 © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e1123 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Alyssa La Bella More articles by this author Alex Molesan More articles by this author Daniel A. Wollin More articles by this author Souvik Paul More articles by this author Ana L. Flores-Mireles More articles by this author Expand All Advertisement PDF downloadLoading ...