Mp15-14 Oncobiome of Urinary Bladder Carcinoma in Indian Males—a Pilot Study

You have accessJournal of UrologyBladder Cancer: Basic Research & Pathophysiology I (MP15)1 May 2024MP15-14 ONCOBIOME OF URINARY BLADDER CARCINOMA IN INDIAN MALES—A PILOT STUDY Manoj Kumar Das, Prasanna Ram, Prasant Nayak, Swarnendu Mandal, Balamurugan Ramadass, and Zainab Khan Manoj Kumar DasManoj Kumar Das , Prasanna RamPrasanna Ram , Prasant NayakPrasant Nayak , Swarnendu MandalSwarnendu Mandal , Balamurugan RamadassBalamurugan Ramadass , and Zainab KhanZainab Khan View All Author Informationhttps://doi.org/10.1097/01.JU.0001009500.87761.bf.14AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Dysbiosis of the urinary microbiome can lead to bladder cancer by producing a chronically inflammatory urothelial microenvironment. Microbes residing inside tumor cells, influencing cancer progression, are known as tumor-associated microbes. Tissue adjacent to tumors is likely to get altered compared to healthy tissue due to immune cell infiltration, fibrosis, and tumor-associated inflammation. Using shotgun metatranscriptomics, we conducted a pilot study to characterize the bladder microbiota in paired tumor and non-tumor mucosa samples of patients with bladder cancer. METHODS: We employed shotgun metatranscriptomics to profile microbes associated with the tumor. Following rigorous inclusion and exclusion criteria, we collected two bits of tissue from consenting patients scheduled for transurethral resection of bladder tumor (TURBT)—one from the tumor site and the other from the normal bladder site. Total RNA was extracted and sequenced, enabling us to identify genes with altered expression profiles between the tumor site and the normal site in the carcinoma bladder. The taxonomy of the microbes was determined using Kracken and Bracken tools. RESULTS: We observed high microbial richness in the normal tissue compared to the cancer site in patients revealing the microbial richness is an indicator of health. The top 20 microbes belonged to the phyla Actinomycetota, Pseudomonadata, Bacillota, and Uroviricota. Comparative analysis of the top 20 species between normal and cancer sites showed microbes such as Cutibacterium acnes, Escherichia coli, Pseudomonas sp. CIP-10, Staphylococcus aureus, Staphylococcus cohnii, Yersinia enterocolitica were found to be more in cancerous sites than normal sites. In contrast, normal sites are prevalent by Klebsiella grimontii, Klebsiella michiganensis, Mycobacterium canettii, Pseudomonas aeruginosa, and Streptomyces lividans. We did not observe a statistically significant difference between these two sites regarding their alpha and beta diversity at the species level. Bifidobacterium adolescentis was observed as an indicator species in normal sites, revealing its importance in tumor suppression. However, patient-wise taxonomy did not show any significant differences. CONCLUSIONS: Identifying the bacterial species involved could be beneficial to understanding and preventing the onset, advancement, and progression of bladder cancer. The urinary microbiome may be a biomarker to help predict the response to BCG therapies. Further, longitudinal studies are needed to assess the role of the bladder microbiota in carcinogenesis. Source of Funding: None © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e235 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Manoj Kumar Das More articles by this author Prasanna Ram More articles by this author Prasant Nayak More articles by this author Swarnendu Mandal More articles by this author Balamurugan Ramadass More articles by this author Zainab Khan More articles by this author Expand All Advertisement PDF downloadLoading ...