Bioactives of Hypericum Perforatum to Control Diabetes: In Vitro and In Vivo Biological Evaluation in Diabetic Rats

first_page Download PDF settings Order Article Reprints Font Type: Arial Georgia Verdana Font Size: Aa Aa Aa Line Spacing:    Column Width:    Background: Open AccessAbstract Bioactives of Hypericum Perforatum to Control Diabetes: In Vitro and In Vivo Biological Evaluation in Diabetic Rats † by Neelam SinghNeelam Singh SciProfiles Scilit Preprints.org Google Scholar 1,* and Puneet GuptaPuneet Gupta SciProfiles Scilit Preprints.org Google Scholar 2 1 Noida Institute of Engineering and Technology (Pharmacy Institute), 19 Knowledge Park II, Institutional Area, Noida 201306, India 2 Amity Institute of Pharmacy, Amity University Uttar Pradesh, Sector 125, Noida 201313, India * Author to whom correspondence should be addressed. † Presented at the 3rd International Electronic Conference on Biomolecules, 23–25 April 2024; Available online: https://sciforum.net/event/IECBM2024. Proceedings 2024, 103(1), 50; https://doi.org/10.3390/proceedings2024103050 Published: 12 April 2024 (This article belongs to the Proceedings of The 3rd International Electronic Conference on Biomolecules) Download keyboard_arrow_down Download PDF Download PDF with Cover Download XML Download Epub Versions Notes Keywords: hypericin; antioxidants; anti-diabetic; pancreas; lipid; renal; hepatic; plant botanicals Background: Improving diabetes mellitus treatment is a significant contemporary medical and societal issue. Plant-based botanicals may lower blood sugar, enhance insulin absorption, and block important enzymes that contribute to the onset and course of diabetes. The preventive role of Hypericin perforatum and its constituent hypericin was studied using diabetic rats and in vitro cultures.Methods: The in vitro glucose absorption by yeast cells, alpha-amylase, and glucosidase activity were used to examine the mechanism of action of Hypericum perforatum (HP) extract. The extract's safety and in vivo efficacy were studied using a Streptozotocin (STZ)-induced diabetic rat model. Hematology, blood biochemistry, and plasma insulin were also assessed.Results: The extract significantly (p0.03) enhanced the uptake of glucose through the plasma membrane of yeast along with α-amylase (p0.05) and α-glucosidase (o.o4) inhibition activity in vitro. Meanwhile, the extract could significantly reduce renal (uric acid and creatinine), hepatic (SGOT, SGPT, and ALP), and lipid (triglycerides and cholesterol) profiles in the serum of diabetic rats. The extract was safe up to a 1000 mg/kg dosage in preclinical acute dose toxicity studies. In diabetic rats, the extract reduced pancreatic histological alterations. This study found that the extract reduced STZ-induced diabetes and oxidative stress to liver and pancreatic tissue, as well as increasing plasma insulin levels in the treated rats.Conclusions: Our findings indicate that Hypericum perforatum extract demonstrated protective activity by effectively halting the sequential progression of oxidative stress, renal and hepatic function test, lipid profile test, and STZ-induced histopathological alterations in the liver and pancreas. Author ContributionsConceptualization, N.S.; methodology, N.S. and P.G.; software, P.G.; validation, N.S. and P.G.; formal analysis, N.S.; investigation, P.G. All authors have read and agreed to the published version of the manuscript.FundingThe research received no external funding.Institutional Review Board StatementPresent study proceeds from the CPCESA-India guidelines, after getting an approval from the Institutional Animal and Ethics Committee (IAEC) at I.T.S. College of Pharmacy (ITS/05/IAEC/2022).Informed Consent StatementNot applicable.Data Availability StatementThe current study data is available from the corresponding author on reasonable request.Conflicts of InterestThe authors declare no conflict of interest.Disclaimer/Publisher's Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Share and Cite MDPI and ACS Style Singh, N.; Gupta, P. Bioactives of Hypericum Perforatum to Control Diabetes: In Vitro and In Vivo Biological Evaluation in Diabetic Rats. Proceedings 2024, 103, 50. https://doi.org/10.3390/proceedings2024103050 AMA Style Singh N, Gupta P. Bioactives of Hypericum Perforatum to Control Diabetes: In Vitro and In Vivo Biological Evaluation in Diabetic Rats. Proceedings. 2024; 103(1):50. https://doi.org/10.3390/proceedings2024103050 Chicago/Turabian Style Singh, Neelam, and Puneet Gupta. 2024. "Bioactives of Hypericum Perforatum to Control Diabetes: In Vitro and In Vivo Biological Evaluation in Diabetic Rats" Proceedings 103, no. 1: 50. https://doi.org/10.3390/proceedings2024103050 Article Metrics No No Article Access Statistics Multiple requests from the same IP address are counted as one view.