The Role of Amyloidogenesis in Cancer Development: An Investigation of Transcription Factors

first_page Download PDF settings Order Article Reprints Font Type: Arial Georgia Verdana Font Size: Aa Aa Aa Line Spacing:    Column Width:    Background: Open AccessAbstract The Role of Amyloidogenesis in Cancer Development: An Investigation of Transcription Factors † by Marina V. RyabininaMarina V. Ryabinina SciProfiles Scilit Preprints.org Google Scholar *, Andrew A. ZelinskyAndrew A. Zelinsky SciProfiles Scilit Preprints.org Google Scholar and Aleksandr A. RubelAleksandr A. Rubel SciProfiles Scilit Preprints.org Google Scholar Laboratory of Amyloid Biology, Saint-Petersburg 190000, Russia * Author to whom correspondence should be addressed. † Presented at the 3rd International Electronic Conference on Biomolecules, 23–25 April 2024; Available online: https://sciforum.net/event/IECBM2024. Proceedings 2024, 103(1), 70; https://doi.org/10.3390/proceedings2024103070 Published: 12 April 2024 (This article belongs to the Proceedings of The 3rd International Electronic Conference on Biomolecules) Download keyboard_arrow_down Download PDF Download PDF with Cover Download XML Download Epub Versions Notes Keywords: amyloids; cancer; transcription factors Cancer remains the leading cause of death due to a wide range of molecular mechanisms. One of these mechanisms is impaired proteostasis, which leads to the formation of amyloids, fibrillar proteins with an intermolecular cross-beta structure. Studies have shown that amyloids and amyloidogenic proteins are associated with tumor progression. For example, elevated levels of SAA amyloid in serum correlates with the development of lung, breast cancer, and melanoma. Similarly, the IAPP protein is associated with neuroendocrine tumors, and ODAM is associated with odontogenic tumors [1]. By using bioinformatics algorithms, we identified the amyloidogenic potential of five transcription factors involved in the pathogenesis of various cancers. Experimental testing of the amyloidogenic potential of these proteins in a yeast assay [2] partially confirmed the in silico data. The goal of this study was to further investigate the amyloid potential of these proteins in vitro. We employed the bacterial C-DAG production and export system [3] and/or purified recombinant proteins from E. coli with metal-affinity chromatography. For transcription factors that demonstrated amyloidogenic potential in bacteria, we analyzed their amyloid-like properties in human HEK293T cell culture. The results obtained in this study provide new insights into human proteins capable of forming amyloids and offer promising prospects for identifying potential targets for cancer therapy. Author ContributionsConceptualization, A.A.R.; data acquisition and formal analysis, M.V.R. and A.A.Z.; supervision, A.A.R.; funding acquisition, A.A.R.; writing—review and editing, A.A.R. and M.V.R. All authors have read and agreed to the published version of the manuscript.FundingThe authors acknowledge Saint-Petersburg State University for research project 95444727.Institutional Review Board StatementNot applicable.Informed Consent StatementNot applicable.Data Availability StatementNo new data were created.AcknowledgmentsThe authors acknowledge the support from Core Facility "Chromas" (Research Park, St. Petersburg State University) for technical assistance.Conflicts of InterestThe authors declare no conflicts of interest.ReferencesKachkin, D.V.; Volkov, K.V.; Sopova, J.V.; Bobylev, A.G.; Fedotov, S.A.; Inge-Vechtomov, S.G.; Galzitskaya, O.V.; Chernoff, Y.O.; Rubel, A.A.; Aksenova, A.Y. Human RAD51 Protein Forms Amyloid-like Aggregates In Vitro. Int. J. Mol. Sci. 2022, 23, 11657. 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Share and Cite MDPI and ACS Style Ryabinina, M.V.; Zelinsky, A.A.; Rubel, A.A. The Role of Amyloidogenesis in Cancer Development: An Investigation of Transcription Factors. Proceedings 2024, 103, 70. https://doi.org/10.3390/proceedings2024103070 AMA Style Ryabinina MV, Zelinsky AA, Rubel AA. The Role of Amyloidogenesis in Cancer Development: An Investigation of Transcription Factors. Proceedings. 2024; 103(1):70. https://doi.org/10.3390/proceedings2024103070 Chicago/Turabian Style Ryabinina, Marina V., Andrew A. Zelinsky, and Aleksandr A. Rubel. 2024. "The Role of Amyloidogenesis in Cancer Development: An Investigation of Transcription Factors" Proceedings 103, no. 1: 70. https://doi.org/10.3390/proceedings2024103070 Article Metrics No No Article Access Statistics Multiple requests from the same IP address are counted as one view.