HEP-TILE: HBV whole genome sequencing (nanopore protocol) v1

This protocol describes the HEP-TILE tiled amplicon protocol for whole genome sequencing of Hepatitis B virus (HBV) on the nanopore MinION. We developed a pan-genotypic (genotypes A-J) HBV scheme using an early version of PrimalScheme3, a web-based primer design tool for developing multiplex primer schemes. PrimalScheme3 is a successor to PrimalScheme, with a number of changes made to enable us to generate an overlapping (tiled) amplicon scheme which covered the circular HBV genome, utilising a number of discrete primers at each position to handle intraspecies diversity. Primer sequences are here: https://github.com/quick-lab/primerschemes/blob/main/primerschemes/hbv/600/v2.1.0/primer.bed The amplicons can also be fragmented and sequenced on Illumina platforms.