Supplementary Figure 3 from Immune Modulation with RANKL Blockade through Denosumab Treatment in Patients with Cancer

Supplementary Figure 3: Expression of immune checkpoints at baseline (A) A uniform manifold approximation and projection (UMAP) plot of cells from all peripheral blood mononuclear cell (PBMC) samples analyzed with Scaffold using data from cytometry by time of flight (CyTOF). Clusters correspond to canonical immune cells and are represented by distinct colors. (B) Expression of PD-L1, TIM3, and CTLA-4 at baseline and post-treatment timepoint 2 (TP2). Raw count of metal ions detected from CyTOF were asinh-transformed, with a higher value corresponding to a higher expression of immune checkpoint.