FIGURE 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate

Determining the immune phenotype of tumor tissues. A total of 26 TNBC tissues were analyzed for the TIP. A, Annotation of slide on QuPath for analysis of the four regions: Stroma, Outer Invasive, Inner Invasive, and Tumor Core. Number of cells per mm² and VCAN percentage were counted in these regions. B, Box plot showing desert tissues under the lower quartile. C, Comparison of cells in the stromal zone and epithelial zone to determine excluded and inflamed tissues. Line indicates a ratio of 0.75. D, Pie charts showing distribution of phenotypes for 26 tissues. Table showing phenotype per tissue for CD8⁺ T cells and CD68⁺ macrophages. E, Pie charts showing phenotypes within each tissue. Graphs comparing levels of CD8⁺ T cells in the epithelial zone (EZ) and stromal zone (SZ).