Supplementary Figure 4 from Oncogenic Cells of Renal Embryonic Lineage Sensitive to the Small-Molecule Inhibitor QC6352 Display Depletion of KDM4 Levels and Disruption of Ribosome Biogenesis

Dose-dependent decrease in ribosomal protein expression in response to QC6352 treatment in WiT49 and HEK293. Treatment of WiT49 and HEK293 cells with the above concentrations of QC6352 for 72 hours resulted in a dose-dependent decrease in KDM4A-C and the ribosomal proteins RPS6, RPS3, RPL7a, RPL26, and RPS27a by western blot. In addition, a dose dependent decrease in P70 S6Kinase, which phosphorylates the ribosomal protein RPS6 to initiate protein translation, was observed.