Supplementary Figure S4 from Neutrophils Mediate Protection Against Colitis and Carcinogenesis by Controlling Bacterial Invasion and IL22 Production by γδ T Cells

Figure S4. Neutrophil deficiency is associated with decreased expression of IL-22 by γδ T cells. Related to Figure 4-5. A-C) Representative gating strategy used to gate myeloid population (A), lymphoid population (B) and innate lymphoid cells (ILC) (C) in colon LP. UTC: unconventional T cells (TCRβ+, CD8-, CD4-). D) IL-22 expression in myeloid cells derived from DSS-treated Csf3r+/+(n=3), Csf3r-/-(n=3) stimulated 4 hours with IL-23 analyzed by FACS. E-F) Expression of IL-22 by lymphoid cells derived from DSS-treated Csf3r+/+(n=4), Csf3r-/- (n=4) stimulated 4 hours with IL-23 plus IL-1β (E) and PMA plus ionomycin (F) analyzed by FACS. A-F) Representative data of three independent experiments. D-F) Multiple t-test. Data are mean ± SEM. *** p < 0.001 ** p < 0.01 * p < 0.05.