Use of cholera toxin subunit B to label neural projections to major pelvic ganglia v1

This protocol is used to visualise preganglionic neurons in the spinal cord innervating pelvic visceral organs (e.g., the lower urinary tract) in an experimental adult male or female rat. The protocol is performed under anesthesia and should incorporate all local requirements for standards of animal experimentation, including methods of anesthesia, surgical environment, and post-operative monitoring and care.