Real-Time PCR Diagnosis of COVID-19 in Al Anbar Province, Iraq

Background: Genomic sequencing has advanced COVID-19 understanding, facilitating therapeutic assessment and guiding treatment strategies. Precise diagnosis is vital for grasping transmission dynamics and detecting novel variants. The study aimed to investigate the molecular attributes, mutations, and diagnostic methodologies of the coronavirus (SARS-CoV-2) pandemic. Methods: Real-time PCR was utilized for COVID-19 diagnosis in Al Anbar province. Samples underwent RNA extraction and RT-PCR amplification. Between February and April 2020, 245 samples were collected from Anbar province, initially yielding negative results via rtPCR. Data on infection rates, gender disparities, and clinical outcomes were analyzed. Results: In 2020, the infection rate was 14%, reducing to 11% in 2021. Men exhibited higher infection rates (70%) compared to women (30%). Clinical data indicated varying severity and fatality rates between sexes. Conclusion: While RT-PCR remains widely accepted, alternative diagnostic methods show promise. Further research is essential for comprehensive COVID-19 understanding and improving diagnostic accuracy. The study underscores ongoing research's significance in combating viral epidemics, emphasizing the need for comprehensive understanding and adaptive strategies to mitigate mortality risks.