The Effects of Limosilactobacillus fermentum IDCC 3901 Cell Lysate and Cell-Free Supernatant on Photoaging in UVB- exposed Human Keratinocytes

Probiotic microorganisms are one of the useful ingredients that can be used in the manufacture of cosmetics and internal beauty products.The aim of this study is to determine the feasibility of using probiotic bacterial culturederived materials as cosmetic materials by measuring the effect on UVB-induced photoaging in human keratinocytes.Considering the formulation design for human application, the bacterial cell lysate and culture supernatant were separated from the cultured broth of Limosilactobacillus fermentum IDCC 3901.The test materials were treated to HaCaT cells and then irradiated with UVB to determine cell viability, antioxidant activity, and inhibition of collagen degradation.Cell survival rate, reactive oxygen species (ROS) production, superoxide dismutase and catalase activity, collagen content, and matrix metalloproteinases (MMPs) production were measured.The test materials demonstrated protective effects on HaCaT cells exposed to UVB, as evidenced by a reduction in ROS generation through enhanced antioxidant enzyme activity.Additionally, the test materials down-regulated the expression of MMP-1 and MMP-9, resulting in an augmentation of collagen content.These findings suggest that materials derived from probiotic cultivation exhibit potential for use as anti-aging agents in cosmetics, particularly against UVBinduced skin damage.