Creating Custom Brain Slicing Matrices with 3D Printers v1

To create reproducible brain sections, brain slicing matrices are often utilized. These matrices are designed with anatomically proportioned cavity dimensions and precisely spaced slice channels, enabling consistent and accurate slicing of the brain into coronal or sagittal sections. Brain matrices can be customized for various animal species or developmental stages, and a wide range of models are commercially available. However, there are certain limitations to the variety of these prefabricated products. In this protocol, we design a brain matrix specifically for rodents, tailored to the measurements of the brain sample, and produce the customized brain slicing matrix with a 3D printer.