Key points are not available for this paper at this time.
This study centers on creating and confirming a specialized liquid chromatography-tandem mass spectrometry (LCMS/MS) bioanalytical technique for accurately measuring the levels of lamivudine, zidovudine, and nevirapine in human plasma. The approach utilizes abacavir as a reference point. The utilization of positive ionization mode, solid-phase extraction (SPE) technology, and the deliberate addition of formic acid in the mobile phase played a crucial role in enhancing the sensitivity of the approach. The purity advance C18 column exhibited superior performance, ensuring favorable peak shapes and responses even at low concentrations. Rigorous method validation, in accordance with international guidelines, confirmed the method’s reliability, meeting acceptance criteria for specificity, linearity, accuracy, precision, and robustness. Successful application to patient samples highlighted the method’s practical utility for routine monitoring and quality control in pharmaceutical and clinical settings. The paper discusses comprehensive findings, suggesting further optimization for simultaneous determination and emphasizing the potential for broader applications in pharmacokinetic studies and therapeutic drug monitoring.
Shelke et al. (Mon,) studied this question.