Figure S8 from Spectral Flow Cytometry Methods and Pipelines for Comprehensive Immunoprofiling of Human Peripheral Blood and Bone Marrow

Figure S8. Pre-integration LISI scores for PBMC and BMC UMAPs. Dot plot indicating proportion of cells with a high Local Inverse Simpson Index (LISI) score in concatenated UMAPs. To calculate this proportion, we set a threshold of LISI > 2 in those cases where scores could range from 1-3 (“BMC” and “PBMC (All Donors)”; UMAPs shown in Fig. 6A and 5A-D), or LISI > 1.667 in those cases where scores could range from 1-2 (“PBMC (Excluding Donor 3146)”, UMAPs not shown). Circular data points represent the proportion of LISI-high cells per indicated donor within each UMAP; red squares indicate the average proportion of LISI-high cells among all donor cells in the respective UMAP. For PBMC UMAPs, “All Donors” represents the inclusion of all three PBMC samples, while “Excluding Donor 3146” excludes a donor who had a B cell lymphoma, demonstrating how biological differences can impact integration.