Antibacterial activity and inhibitory concentration of hydrolysis protein of Rainbow trout (Oncorhynchus mykiss) waste on Exotoxin A Gene Expression of Pseudomonas aeruginosa

Pseudomonas aeruginosa is a harmful pathogen known for causing various infections. A recent study aimed to explore the antibacterial properties of Rainbow trout (Oncorhynchus mykiss) waste protein hydrolysate against P. aeruginosa. Antibacterial peptides from fish hydrolyzed protein (FPH) were tested using disk diffusion and well diffusion methods, with a focus on the inhibitory concentration (IC50) of FPH less than 3 kDa on P. aeruginosa ATCC 27853's exotoxin A gene expression. After exposing P. aeruginosa to FPH less than 3 kDa in BHI broth medium and analyzing gene expression of exotoxin A using Real-Time PCR, it was found that the inhibitory concentration had a significant impact. The minimal inhibitory concentration (MIC) of FPH less than 3 kDa against P. aeruginosa was determined to be 0.12 μg/mL. These findings suggest that FPH can serve as an effective antimicrobial agent, potentially preventing toxin production and enhancing the safety of perishable foods.