Determination of Cucurbitin B in PCL-g-PEI Micelle by HPLC

Objective: To establish a method for the determination of Cucurbitin B in drug-loaded PCL-g-PEI polymer micelles. Methods: Cucurbitin B loaded micelles were prepared by solvent volatilization. HPLC method was used to determine the content of Cucurbitin B in PCL-g-PEI micelles. The separation was performed on Agilent C18 column (150 mm×4.6 mm, 5 μm) with mobile phase consisted of acetonitrile/water/glacial acetic acid=45: 55: 0.1 (V/V/V) at the flow rate of 1.0 mL/min. The detection wavelength was set at 228 nm, and the column temperature was 25°C. Sample volume was 20 μL. Result: The good linearity with Cucurbitin B concentration range was achieved in the rang of 1×10⁻⁶~0.1 mg/mL (r=0.9999). Blank PCI-g-PEI micelle carrier had no interference. The precision, stability, repeatability and recovery of the method meet the requirements. Solvent volatilization method was employed to prepare Cucurbitin B drug-loaded micelle, which has the highest drug loading capacity among them. Conclusion: The method for the determination of Cucurbitin B established in this paper has strong specificity, good precision and reproducibility, and the recovery rate meets the requirements. The content of Cucurbitin B in drug-loaded polymer micelles can be quantitatively determined, which provides reference for the content determination of Cucurbitin B related preparations of Traditional Chinese medicine.