Answers are grounded in this paper and cite their sources.
This protocol was modified from the QIAGEN Plant Mini Kit Handbook to extract fungal DNA from within the seed coat of small (<1mm diameter) seeds. It can be followed by amplification with fungal specific primers (ITS-1F KY02 and ITS-4 KY01) to optimize concentration of fungal DNA. Together, this maximizes the ratio of fungal DNA to plant DNA for metagenomic analysis of the fungal community.
No takes yet. Share an insight, caveat, or question.
Eve Kenny (2024) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: