Immunofluorescent (IF) microscopy is a powerful tool used in cellular and molecular biology to monitor the subcellular localisation of proteins. By combining the advantages of immunostaining and confocal light microscope, IF microscopy can be used to assess the colocalization of two or more proteins within the cell. Here, we describe a method that can be used to verify the correct localisation of endogenously expressed TMEM192, by assessing their colocalization with LAMP1 (a lysosomal marker) and ATPB1 (a mitochondrial marker). Furthermore, our data showed that the anti-TMEM192 antibody is compatible for immunofluorescence assay.
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Rotimi Fasimoye (2024) studied this question.
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