Antibiotic susceptibility testing by infrared multispectral imaging : identification of optimal wavelengths

Antibiotic resistance is a critical public health concern requiring fast, affordable, and reliable diagnostic methods. This study focuses on identifying optimal wavelengths for multispectral imaging in antibiotic susceptibility testing. Deuterium isotope probing and FTIR spectroscopy were used to analyze the metabolic impact of antibiotics on bacteria. Characteristic wavelengths indicating variations in bacterial metabolism were identified. This approach holds promise for expedited antibiotic sensitivity assessment, potentially delivering results within two hours. The utilization of multispectral imaging presents a cost-effective and innovative tool for bacterial identification and combating antibiotic resistance.