On-chip chemiluminescence biosensor for detection of surface contamination

A novel on-chip chemiluminescence biosensor is developed to assess cleanliness of the solid surfaces. The sensor is based on ATP-mediated chemiluminescence detection. It provides high-sensitivity detection of surface contamination due to improved photon collection efficiency. The results show the dependence of the bioluminescence enhancement on ATP concentration. We report the limit of detection of the biosensor. We demonstrate the use of the biosensor for real sample analysis to assess surface contamination of the laboratory and office equipment. This suggests the use of the biosensor in practical applications such as the food processing industry, laboratory environments, and health care settings.