NEBNext® Poly(A) mRNA Magnetic Isolation Module NEB #E7490S/L (Standard Protocol) v1

The NEBNext Poly(A) mRNA Magnetic Isolation Module is designed to isolate intact poly(A)+ RNA from previously isolated total RNA. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour. The new protocol for poly(A) mRNA enrichment (Section 1, Express Protocol) enables comparable performance to our current protocol (Section 2, Standard Protocol) using a substantially faster and more streamlined workflow.