Single-shot laser speckle mirco-rheology using a rolling shutter camera

We present a single-shot laser speckle optical micro-rheology technique that measures tissue viscoelasticity from a single elongated speckle image, avoiding the need for high-speed cameras used in traditional optical micro-rheology. By calculating the intensity autocorrelation between rows of a rolling shutter speckle image with different inter-row delays, we can extract the decorrelation time of dynamic speckles, which directly related to the viscoelastic modulus of multiple scattering samples. Our method extends the range of measured viscoelastic modulus in optical micro-rheology by capturing speckle images with equivalent frame rates over 100,000 frame per second. This enables precise viscoelasticity assessments of diverse biological tissues and tissue phantoms.