Accelerating two-photon fluorescence imaging rates and spectral throughput using arrays of single photon sensitive detectors

Most two photon and confocal microscopes are limited by the sequential detection of pixels. I present recent work using low-cost silicon photomultiplier (SiPM) technology to build arrays of single-photon sensitive detectors. I present both spatially multiplexed imaging in highly scattering tissue and hyperspectral detection using 16 parallel SiPMs in two detector configurations. These enable 200 MP/s imaging through 4 spectral channels or hyperspectral read out at 50 MP/s and 20 nm spectral resolution at very low cost.