Optical coherence tomography assisted motion-corrected Brillouin microscopy of skin in vivo

Biomechanical testing of human skin in vivo is important for studying the aging process and pathological conditions such as skin cancer. Brillouin microscopy allows non-contact measurements of the bulk elastic modulus of the skin, providing information on the mechanical properties of the tissue. Here, we use the combination of Brillouin microscopy and OCT imaging for motion-corrected, depth-resolved biomechanical testing of human skin in vivo. We found that the experimentally measured Brillouin frequency shift of the epidermis is higher than that of the dermis, indicating the higher stiffness of the upper skin layer.